基本信息

个人简历

职 务

现从事专业及研究方向

主要业绩

发表论文

基本信息

单位：温州医学院检验医学院/生命科学学院

性别：男

出生年月：1964年2月

毕业院校：第四军医大学

学历：博士研究生

学位：博士

职称：教授、博士生导师

个人简历

教育经历

1981.08-1986.07 第一军医大学军医系，获学士学位

1986.08-1989.07 第一军医大学生物化学专业，获硕士学位

1991.08-1994.07 第四军医大学微生物学专业，获博士学位

工作经历

1989.08-1991.07 第一军医大学生物化学教研室助教

1991.08-1994.11 第一军医大学免疫学教研室讲师

1994.12-1998.03 第一军医大学生物技术中心副教授

1995.01-1996.01 加拿大卡尔加里大学（University of Calgary）博士后

1996.02-1997.01 美国托马斯.杰弗逊大学（Thomas Jefferson University）博士后

1997.02-2000.07 美国德州大学西南医学中心（UT Southwestern）博士后

2000.08-2005.02 美国德州大学西南医学中心（UT Southwestern）讲师

2005.03-2007.03 南方医科大学生物技术学院生物治疗研究所教授，主任

2007.03-至今 温州医学院生命科学学院细胞与分子医学研究所教授，主任

职 务

钱江特聘教授、教育部检验医学省部共建重点实验室免疫学方向负责人、浙江省模式生物技术与应用重点实验室主任、国家精品课程《免疫学与免疫学检验》的主持人、浙江省高等学校教学名师、温州医学院科技处处长、生命科学学院细胞与分子医学研究所主任

美国生物物理学会会员

现从事专业及研究方向

生物技术、免疫学，肿瘤等重大疾病的免疫学诊断与治疗研究

主要业绩

曾获国家II类新药证书一个，军队科技进步二等奖、广东省科技进步二等奖各一项，拥有发明专利三项、已申报发明专利12项。出国留学前，曾承担过国家863分题、国家自然科学基金、广东省重大项目、军队和广东省自然科学基金各一项。发表SCI研究论文30余篇，其中作为第一或责任（含共同责任）作者发表SCI论文14篇。在生物技术制药领域，取得了如下成绩： 1. 作为主要研发者成功研制了“重组人粒细胞集落刺激因子注射液”（于1998年获新药证书），并实现产业化。该产品1998年列入国家火炬计划，2000年被评定为江苏高新技术产品，已实现利税近亿元。

2.利用细胞膜表面蛋白质的伯氨易生物素化以及生物素与链亲和素的高效超强结合——这两个特性，发明了一种快速、高效、安全的细胞膜表面锚定修饰技术（863计划课题号2006AA02Z4C4申请发明专利9项，已授权1项；发表研究论文18篇，其中SCI收录5篇），并发展了一种防治表浅膀胱癌术后复发、安全有效的新型免疫疗法（现正在申报临床研究批文）。该平台还可适用于其它肿瘤，有望形成有自主知识产权的新型膜表面修饰肿瘤疫苗产业群。

3. 利用脂联素球部可自发聚集形成三聚体的特性，将其与可溶性肿瘤坏死因子受体II连接形成双功能融合蛋白,研制成功了一种新型高效的TNFα拮抗剂(申请发明专利2项，已授权1项), 有可能成为治疗类风湿性关节炎、肥胖症、II型糖尿病、冠心病和脂肪肝的新药。

现主持省部级以上科研课题6项：国家“重大新药创制”科技重大专项项目“可溶性肿瘤坏死因子受体II-脂联素球部融合蛋白的开发研究”(2009ZX09103-649) 、国家自然科学基金重大研究计划培育项目 “连接B[a]P/B[a]PDE诱导的肺慢性炎症微环境至肺癌发生发展的分子机制” （9102970）、 国家自然科学基金面上项目(30971516)“ JNK1经VHL-非依赖途径调节HIF-1α蛋白降解的新功能及其机制研究” (30971516)、国家自然科学基金海外及港澳学者合作基金“IKK/NFκBp50/GADD45α凋亡通路的分子基础”(30928023，中方负责人)等。

发表论文

1. Zhang F, Wu J, Xu C, Lin X, Zhao H, Lu J, Zhang Y, Lu J, Zhang X, Ma J, Shu Y, Lou Y,Gao J*, Wang Y*. Infectivity of pseudotyped particles pairing hemagglutinin of highly pathogenic avian influenza a H5N1 virus with neuraminidases of the 2009 pandemic H1N1 and a seasonal H3N2. Journal of Bioterrorism & Biodefense 2011 (in press)

2. Zhang Z, Xu X, Zhang X, Chen X, Chen Q, Dong L, Hu Z, Li J,Gao J*.The therapeutic potential of SA-sCD40L in the orthotopic model of superficial bladder cancer. Acta Oncologica 2011 Jan 19. [Epub ahead of print]

3. He Q, Li J, Yin W, Song Z, Zhang Z, Yi T, Tang J, Wu D, Lu Y, Wang Z, Liu D, Zhang X, Hu Z, Gao J*. Low-dose paclitaxel enhances the anti-tumor efficacy of GM-CSF-surface-modified whole-tumor-cell vaccine in mouse model of prostate cancer. Cancer Immunol Immunother 2011;60:715-30.

4. Chen Y, Nie X, Cui X, Wu W, Zhang J, Wen C,Gao J* and Jinxin Lu*. Direct Colorimetric Biosensors from Polydiacetylenes. Current Organic Chemistry 2011;15: 518-533.

5. Wen B, Deng Y, Guan J, Yan W, Wang Y, Tan W, Gao J*. Signal peptide replacements enhance expression and secretion of hepatitis C virus envelope glycoproteins. Acta Biochim Biophys Sin (Shanghai). 2011 ;43:96-102.

6. Wu W, Hu H, Li F, Wang L, Gao J, Lu J, Fan C. A graphene oxide-based nano-beacon for DNA phosphorylation analysis. Chem Commun (Camb).2010;47:1201-3.

7. Wu J, Zhang F, Wang M, Xu C, Song J, Zhou J, Lin X, Zhang Y, Wu X, Tan W, Lu J, Zhao H, Gao J, Zhao P, Lu J, Wang Y. Characterization of Neuraminidases from the Highly Pathogenic Avian H5N1 and 2009 Pandemic H1N1 Influenza A Viruses. PLoS One. 2010;5:e15825.

8. Wei B, Gao J, Huang XP, Ji G, Jin JP. Mutual rescues between two dominant-negative mutations in cardiac troponin I and cardiac troponin T. J Biol Chem. 2010; 285:27806-16.

9. Zhou Y, Huang Q, Gao J, Lu J, Shen X, Fan C.A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection. Nucleic Acids Res. 2010; 38(15):e156.

10. Huang X, Yu HS, Chen Z, Li JL, Hu ZM,Gao J*. A novel immunotherapy for superficial bladder cancer by the immobilization of streptavidintagged bioactive IL2 on the biotinylated mucosal surface of the bladder wall. Chinese Journal of Cancer 2010; 29:611-6.

11. Yin W, Hu Z, He Q, Chen Z, Mao Q, Song Z, Qu Z,Nie Xi, Li J, Gao J*. A novel therapeutic vaccine of GM-CSF/TNFα surface-modified RM-1 cells against the orthotopic prostate cancer. Vaccine 2010; 28: 4946-4953.

12. Zhang D, Li J, Costa M, Gao J*, Huang C*. JNK1 mediates degradation HIF-1alpha by a VHL-independent mechanism that involves the chaperones Hsp90/Hsp70. Cancer Res. 2010; 70:813-23.

13. Hu Z, Tan W, Zhang L, Liang Z, Xu C, Su H, Lu J, Gao J*. A novel immunotherapy for superficial bladder cancer by intravesical immobilization of GM-CSF. J Cell Mol Med. 2010; 14:1836-44.

14. Zhang D, Gao J*, Huang C*. c-Jun/AP-1 pathway-mediated cyclin D1 expression participates in low dose arsenite-induced transformation in mouse epidermal JB6 Cl41 cells. Toxicol Appl Pharmacol. 2009; 235(1):18-24.

15. Zhang D, Li J, Song L, Ouyang W, Gao J*, Huang C*. A JNK1/AP-1-Dependent, COX-2 Induction Is Implicated in 12-O-Tetradecanoylphorbol-13-Acetate-Induced Cell Transformation through Regulating Cell Cycle Progression. Mol Cancer Res. 2008; 6(1):165-74.

16. Ouyang W, Luo W, Zhang D, Jian J, Ma Q, Li J, Shi X, Chen J, Gao J, Huang C. PI-3K/Akt pathway-dependent cyclin D1 expression is responsible for arsenite-induced human keratinocyte transformation. Environ Health Perspect. 2008; 116(1): 1-6.

17. J. Gao*, S. Huang, M. Li, R. Luo, X. Wang and A. Takashima. GM-CSF- modified mouse B16.F10 melanoma whole cell vaccine. Vaccine 2006; 24: 5265-5268.

18. N. Mizumoto, J. Gao, Y.Ogawa1, H. Matsushima and A. Takashima. Discovery of novel immunostimulants by dendritic cell-based functional screening. Blood 2005: 106:3082- 3089.

19. Z. Urban, J.Gao, F.M. Pope and E.C. Davis. Autosomal dominant Cutis Laxa with severe lung disease: Synthesis and matrix deposition of mutant tropoelastin. Journal of Investigational Dermatology 2005; 124: 1193-1199.

20. J. Gao and A. Takashima. Cloning & Characterization of Actin, Tr 2 and Tr 4 genes of Trichophyton rubrum. Journal of Clinical Microbiology 2004, 42: 3298-3299.

21. J.P. Jin, D. Wu, J. Gao, R. Nigam and S. Kwong. Expression and purification of the h1 and h2 isoforms of calponin. Protein Expression and Purification 2003; 31: 231-39.

22. C.E. Patterson, J.Gao, Alejandro P. Rooney and E.C. Davis. Genomic organization of mouse and human 65 kDa FK506-binding protein (FKBP65) genes and evolution of the FKBP multigene family. Geneomics 2002; 79: 881-889.

23. J. Gao, L. Waber, M.J. Bennett, K.M. Gibson and J.C. Cohen. Cloning and mutational analysis of human malonyl coenzyme A decarboxylase. J. Lipid Research. 1999; 40:178-182.

24. G.L. Vega, J. Gao, T.P. Bersot, R.W. Mahley, R. Verstraaete, S.M. Grundy, A. White and J.C. Cohen. The -514 polymorphism in the hepatic lipase gene (LIPC) does not influence androgen- mediated stimulation of hepatic lipase activity. Journal of Lipid Research 1998, 39: 1520-1524.

25. Li S. Choksi S. Shan S. Hu X. Gao J. Korngold R. Huang Z. Identification of the CD8 DE Loop as a Surface Functional Epitope: Implication For MHC Class I Binding and CD8 Inhibitor Design. J. Biolo. Chemistry.1998; 273:16442-16445.

26. S. Huang, X Linlai, X Fang, J. Gao, X. Wang and X Liu. Cloning and sequencing of huamn IL2-GM-CSF fusion gene. Chinese J. Cell Mol. Immunol. 1998, 14: 83-85.

27. X Fang, J Gao, X Linlai , M Li and X Wang. Recombination and Identification of granulocyte colony-stimulating factor (hG-CSF) structural gene. Chinese Journal of Immunology 1998;14:375-377.

28. X.Fang, L. Ma, J. Gao, S. Huang and X. Wang. Recombination and Identification of granulocyte colony-stimulating factor in E. coli. Chinese Journal of Biochemical Pharmaceutics. 1998;19: 1-5.

29. T. Satoh, J. Aramini, S. Li, T. Friedman,J. Gao, A. Edling, R.Townsend,U. Koch, S. Choksi, M. Germann, R. Korngold and Z. Huang. Bioactive peptide design based on protein surface epitopes. A cyclic heptapeptide mimics CD4 domain 1 CC' loop and inhibits CD4 biological function. J. Biol. Chem.1997; 272:12175-12180.

30. S. Li, J. Gao, T. Satoh, T. Friedman, A. Edling, U. Koch, S. Choksi, X. Han, R. Korngold and Z. Huang. A computer screening approach to immunoglobulin superfamily structures and interactions: discovery of small non-peptidic CD4 inhibitors as novel immunotherapeutics. Proc. Natl. Acad. Sci. USA 1997; 94:73-78.

31. X Fang, X Linlai, J Gao, and X Wang. Purification and characterization of recombinant huamn G-CSF expressed in E. coli. Chinese Journal of Biologicals. 1997;10: 1-4.

32. X Fang, J Gao , M Li, J Zhou, S Huang, Z Chen and X Wang. Expression of human G-CSF structural gene by using a two-plasmid system. Acta. of the First Medical Collegeof PLA. 1997; 17: 253-257.

33. J. Gao, J.M. Hwang and J.P. Jin. Complete nucleotide sequence and structural organization of mouse h1-calponin gene. Biochem. Biophys. Res. Commun. 1996; 218: 292-297.