简介

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简介

目的研究不同偶联模式的兴奋性氨基酸受体所介导洲爪蟾卵母非细胞跨膜电流的相互影响。方法用异硫氰酸胍-酚-氯仿法从成年大鼠脑中提取总RNA,以寡脱氧胸苷酸纤维素亲和层析法分离出mRNA并注射入非洲爪蟾卵母细胞使表达,通过全细胞双电极电压钳位法,分别以M-胆碱受体Carb,谷氨酸离子型受体激动剂kainate（KA）,代谢Ⅰ型受体激动剂quisqualate（QA）及代谢Ⅲ型受体激动剂L-phosphoserine（L-SOP）两两同时灌流有受体表达的非洲爪蟾卵母细胞。结果3种偶联模式的受体激动后产生的膜电流具有交叉脱敏现象。结论在多种受体共存的细胞中,受体之间可能存在着广泛的相互作用。

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