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脚印拓取，俗称“踩点”。是黑客入侵预定目标如服务器检测目标有何漏洞搜集有用信息资讯再最终制定入侵方案的方法之一，另一种一般为扫描.

footprinting开始要确定侵入的区域和目标。一旦了解了这些信息，那么关于该组织的特定资料就可以用不进入的方式来收集到。例如，该组织自有的网页也许提供了员工的姓名地址录或个人简历，如果电脑黑客需要使用社交手段来达到某种目的，那这些就非常有用。获得的其它信息可能包括其使用的网络配制，从属的公司或实体，联系方式，联系人或邮箱地址，安全政策，相关连接等。

足迹法

Footprinting Procedures

· DNase I Footprinting (Mike A. Dyer)

· DNase I footprinting

Determining the site of binding for a protein on a DNA sequence

· DNase I Footprinting (Bowtell Lab)

· DNase I Footprinting (Crawford Lab)

Detailed protocol for footprinting, including strategy for probe labeling, recipes and more...

· Exonuclease III footprinting

· Hydroxyl radical footprinting (TTO)

(To view this article, registration is needed, but free)

Hydroxyl radical footprinting is a powerful technique for investigating DNA protein interactions. In contrast to DNase I footprinting, it allows the exact determination of contact sites in the DNA target sequence. This is a modification of this method in which the DNA fragments for the footprint assay are non-radioactively labeled and generated by PCR. The fragments are analyzed after incubation with the specific DNA-binding protein and subsequent treatment with hydroxyl radicals, on an ALFexpress DNA Sequencer

Other procedures

· DMS-treating DNA (Indiana U)

DMS treatment under controlled conditions, followed by piperidine cleavage, yeilds a ladder of fragments indicating the positions of all G residues in the DNA sequence. These ladders are useful MW standards for S1 experiments, footprinting, etc.

· Preparation of G+A Marker (Technical Tips Online)

A rapid one-step procedure for preparation of G plus A sequence markers by acid-induced apurinization and DNA cleavage

Preparation of G+A Marker

Simplified method for preparing G+A ladder run along with footprinting reaction. It's much simple than the original Maxima-Gilbert sequencing reaction and works fine.