| 词条 | protein |
| 释义 | § 简介 A Y2Hassay for a protein you don't know ? You will have to do a complete screen of kinases. 蛋白質是一種複雜的有機化合物。蛋白質是由胺基酸分子呈線性排列所形成，相鄰胺基酸的羧基和氨基通過肽鍵連接在一起。蛋白質的胺基酸序列是由對應基因所編碼。除了遺傳密碼所編碼的20種「標準」胺基酸，在蛋白質中，某些胺基酸殘基還可以被翻譯後修飾而發生化學結構的變化，從而對蛋白質進行激活或調控。多個蛋白質可以一起，往往是通過結合在一起形成穩定的蛋白質複合物，發揮某一特定功能。 § 说明 Firstdo some bioinformatics and lit. survey to determine what kind of domains/phosphates your protein has. Unless you provide some details of your protein, this question is a broad as hell. Anyway, if you have an assay for your proteins function (which is presumably phosphorylation dependent), you can usesiRNA against various kinases ans check for LOF of your protein. this might give you a few hits, though you may cause LOF without direct interaction of kinase and protein. After you get hits, you can do binding study/colocalization or Y2H screen . |
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