个人简介

个人经历

研究方向

近五年代表性论文

个人简介

山东大学教授，博士生导师，分子技术研究室主任。近十年来，从事丝状真菌分子遗传学与分子生物学研究工作，先后承担和参加国家与省部级等项目16项，在国内外学术刊物上发表论文50余篇。获奖2次。

个人经历

1999.09至今，山东大学微生物技术国家重点室教授

1992.09-1999.09，山东大学微生物技术国家重点室副教授

1996.1～9月,芬兰技术中心生物技术研究所,从事丝状真菌分子生物学研究。

1993.4～5月; 1991.5～12月, 两次赴澳大利亚悉尼大学农化系进行合作研究。

1982 年毕业于山东大学 生物系 微生物专业。

社会兼职：中国微生物学会分子生物学与生物工程专业委员会委员

研究方向

1 丝状真菌分子遗传学与分子生物学

2 木质纤维素降解代谢酶分子生物学

3 海洋低温适冷酶的酶学与分子生物学

科研成果

承担和参加了16项国家和省部级有关丝状真菌遗传与分子生物学，木质纤维素降解代谢酶分子生物学方向研究课题，在国内外学术刊物上发表论文50余篇，其中SCI论文13篇。研究成果荣获：

“纤维素、半纤维素降解代谢酶分子生物学”获研究山东省科委科技进步三等奖（1999），首位。

参加“微生物对木质纤维素降解机理的研究” 获国家教育部基础类一等奖（1999）。

近五年代表性论文

1.Novel Cold-adaptive Penicillium Strain FS010 Secreting Thermo-labile Xylanase Isolated from Yellow Sea. Acta Biochim Biophys Sin (Shanghai). 2006, 38(2):142-9.

2.Antisense Inhibition of Xylitol Dehydrogenase Gene, xdh1, from Trichoderma reesei Letters in Applied Microbiology, 2005, 40(6):424-9.

3 Cloning and expression of the endogucanase gene from cold-adapted bacteria Pseudoalteromonas sp.MB-1. Acta Microbiologica Sinica 2005，1：142－144

4 Novel cellulase profile of Trichoderma reesei strains constructed by cbh1gene replacement with eg3 gene expression cassette. Acta Biochemica et Biophisica. 2004, 36(10):667-672

5.Construction of a yeast one-hybrid system with the xylanase2 promoter from Trichoderma reesei to isolate transcriptional activators. Letters in Applied Microbiology, 2004, 38(4):277-282

6.Purification and characterization of cold-active α-amylase excreted by a strain of marine cold-adaptive Penicillium, Chemical Research in Chinese University, 2004,20(1): 60-64

7 Construct of the recipient strains for the heterologous gene expression system of Trichoderma reesei. MYCOSYSTEMA 2004, 23（2）：248－254

8 preliminary analysis of the function region deletion in xylanase II promoter in Trichoderma reesei Jounal of ShanDong university(Natural Science) 2004，39（2），102-105

9 Construcion of heterogeneous gene expression system of filamentous fungus Trichoderma reesei. Chinese Jounal of Biochemistry and Molecular Biology 2003，19(6): 736－742

10 Molecular characterization of extreme enzymes Isolated from marine exremophiles. Science ＆Technology Review 2004，9：7－9

11.Cloning and sequence analysis of a mitochondrial gene cluster encodingtwo NADH dehydrogenase subunits and seven tRNA molecules from Trichoderma reesei. Biotechnology Letters 2002, 24:395-399

12.Cloning and sequence analysis of a mitochondrial gene cluster encoding cytochrome C oxidase subunit III from Trichoderma pseudokoningii. DNA Seq. 2002, 13(5): 271-5.

13.Gene Replacement in Trichoderma reesei to Produce Strains with Novel Cellulase Profiles. Biotechnology 2000, The World Congress on Biotechnology, Book of Abstracts Vol. 2, p. 106, Sept. 3-8, 2000, Berlin.

14.Cold adaptation of a mesophilic cellulase, EG III from Trichoderma reesei, by directed evolition. 2001, Science in China, 45(4):337-343

15.Cellulose-binding domain of engoglucanase III from Trichoderma reesei disrupting the structure of cellulose. Biotechnology Letters 2001. 23: 711-715

16.Non-hydrolytic disruption of crystalline structure of by cellulose by cellulose binding domain and linker sequence of cellobiohydrolase I from Penicillium janthinellum. Acta biochimica et biophysica sinica 2001, 33(1):13-18